In order to fast track and compliment conventional maize breeding programme designed for low nitrogen (N) environments. seeds of eight low soil N-tolerant maize lines were sown in the field under no nitrogen and 40 kgN/ha for in vivo evaluation with a view to develop simple protocols for screening maize lines for low nitrogen-efficient through tissue culture technique. Maize plants us... https://www.fitnessgravesyardes.shop/product-category/cam/