Single-molecule and bulk biophysical approaches to study protein-DNA interactions on surface-immobilized nucleic acid templates typically rely on modifying the ends of linear DNA molecules to enable surface-DNA attachments. Unless both strands are constrained. this results in topologically free DNA molecules and the inability to observe supercoiling-dependent biological processes or r... https://www.diegojavierfares.com/amazing-find-Dinah-Lace-Front-Synthetic-Wig-by-Bobbi-Boss-limited-save/