A method using UV-Vis spectroscopy and multivariate tools for simultaneous determination of glucose and cholesterol was developed in this paper. The method is based on the development of the reaction between the analytes (cholesterol and glucose) and enzymatic reagents. The spectra were analyzed by partial least squares regression and artificial neural networks. https://www.spidertattooz.com/Powell-Mahoney-Bloody-Mary-Mix-750ml/